Vanderbilt Study Explains Disproportionate Illness and Deaths Among Young Adults During H1N1 Pandemic Flu
On November 19, Jason Martin returned to the
Medical Intensive Care Unit (MICU) at Vanderbilt University Medical Center for
the first time since he nearly died there during last year's H1N1 flu pandemic.
The tall and burly Warren County, TN, ambulance worker - a 30-year-old, father
of three young children - broke down and hugged some of the nurses he
"I got sick on September 12 and didn't come out of it for the next 20 days. I
am just so grateful I came through," Martin said, wiping his eyes.
Martin was among the first wave of critically ill middle Tennesseans, hit hard
by the H1N1 flu pandemic in late 2009. A hallmark of pandemic flu throughout
history, including the H1N1 pandemic, has been its ability to make healthy
young and middle-aged adults seriously ill and even kill this population in
In a paper published Dec. 5 in Nature Medicine, Fernando Polack, M.D.,
the Cesar Milstein Associate Professor of Pediatrics at Vanderbilt, and
colleagues in both Argentina and Nashville provide a possible explanation for
this alarming phenomenon of pandemic flu. The study's findings suggest people
are made critically ill, or even killed, by their own immune response.
"Every time there is an influenza pandemic there is a large proportion of
younger, or middle-aged adults who die. We have always explained these deaths,
based on presumed virulence of virus, or getting bacterial infection at the
same time. We now have vaccines and antibiotics, but still we see middle-aged individuals
who die," Polack said.
Polack directs the INFANT Foundation, a research and clinical institute based
in Buenos Aires, in close cooperation with Vanderbilt's Vaccine Center. In
Argentina, he had a front row seat for the emergence of the H1N1 flu pandemic,
which began in April 2009.
As the H1N1 virus burned its way northward through the southern hemisphere,
Polack and his team went to work looking for evidence of a biomarker he had
used before. A biomarker is a protein that can be measured in blood or tissue
whose concentration reflects the severity or presence of some disease state.
"We have seen this before. Where non-protective antibody responses are
associated with an immune-based disease in the lung," Polack said.
Polack has previously published evidence that a first-line immune response,
primed by an imperfect antibody, can overreact in a violent and uncontrolled
fashion. Patients die from lung damage inflicted by their own immune system. A
molecule called C4d, a product of this biochemical cascade (the complement
system), is a marker for the strength of the response.
In adults who died during the 2009 H1N1 pandemic, high levels of C4d in lung
tissues suggest a massive, potentially fatal activation of the complement
Pulmonary and critical care physician, Todd Rice, M.D., assistant professor of
Medicine at VUMC, has seen people killed by the "exuberant" and uncontrolled
response of the immune system in other diseases - like sepsis.
"This looked every bit like that," Rice recalled about H1N1 patients, including
Jason Martin, who piled into his intensive care unit in mid-September, 2009.
"It was impressive. These were as sick as any patients I had ever seen. We
tried all sorts of things," Rice said.
Ultimately, Vanderbilt's ICU saw 49 adults with H1N1, most ages 25 to 45. Jason
Martin was one of 40 who survived.
But Polack wanted evidence beyond what he saw in patients in 2009. He asked
Joyce Johnson, M.D., professor of Pathology with VUMC, to look for evidence
this phenomenon may have happened during past flu pandemics. Johnson scanned
Vanderbilt's hand-penned autopsy ledgers, dating back to 1925. She was able to
locate tissue samples in Vanderbilt's tissue archive and extract half a dozen
slices of lung tissue from Nashville patients who died during the 1957 Hong
Kong flu pandemic.
Pediatric infectious diseases expert, John Williams, M.D., assistant professor
of Pediatrics, Microbiology & Immunology, and his lab found the signature
for an influenza infection in 4 of the 53-year-old samples, and were able to
confirm the lung tissues had high levels of C4d. These patients too had died
from an over-reactive immune response.
"C4d is part of the inflammatory cascade, and while it's really good at killing
organisms and protecting us, it's sort of the slash and burn approach, capable
of causing lots of tissue damage," Johnson said.
But why did infants and the frail elderly escape this mechanism of death in the
"We found in 2009, the elderly had good immunity because they had seen a very
similar virus sometime before 1957. Babies hadn't seen many viruses at
all so there was no trigger. It came down to the young adults - primed with an
ineffective response. Their bodies already had defenses against previous influenza
viruses that look like this one but weren't close enough," Polack said.
While the patient sample in the current study is relatively small: 75 patients,
including 23 who died in 2009, and 4 from 1957, investigators were able to show
that other theories, like dampening of interferon, or the triggering of an
inflammatory response called a "cytokine storm," were not supported by the
evidence. Even the number of viruses present in a flu patient did not seem to
correlate with the severity of illness.
"It suggests this (immune over-reaction) is what happens with pandemic to make
young healthy adults seriously ill. There are other things that contribute to
threat, but this is one of the main things for this age group," Polack said.
While many questions remain, one thing is clear: the H1N1 vaccine offers
protection. Patients who died were overwhelmingly unvaccinated. Many fell ill
before a vaccine was even available.
A study of genetics will be among the next steps to explain responses
experienced by people like Jason Martin. It may be possible to find out who is
susceptible to this over-reactive response so they can actively avoid exposure,
or receive a specific regimen of treatment. But until then, vaccination is the